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Ameroid环置入联合高脂饮食诱导致兔单侧颈总动脉粥样硬化性闭塞模型的建立

, PP. 662-666

Keywords: 颈动脉疾病,,收缩环,超声检查,多普勒,彩色

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Abstract:

?目的?对高脂饮食喂养的新西兰大白兔联合Ameroid环置入建立新西兰大白兔单侧颈总动脉粥样硬化性闭塞模型,探讨颅外段动脉粥样硬化性闭塞所致的血流动力学改变与脑组织微血管及超微结构改变的表现。方法??雄性新西兰大白兔20只,随?机分为正常对照组(Ⅰ组)和高脂喂养组(Ⅱ组),每组10只。Ⅱ组经高脂喂养4周后取其中6只行右侧颈总动脉Ameroid环置入术,分别?于置环后第1、2、3、4周末行颈总动脉内膜中层厚度(IMT)及血流动力学参数检测,对照组在同时间点行相同指标的检测。术后第4?周末,取脑组织行免疫组化检测和电镜观察。结果??Ⅰ组各项检测指标与术前相比均无明显差异(P>0.05)。Ⅱ组右侧颈总动脉置环术后血流束逐渐变细,血流速度增高,第4周血流速度降低,4周末时无血流显示。频谱显示两组血流动力学参数差异显著(P<?0.05)。免疫组化检测可见Ⅱ组术后脑组织新生微小血管较I组显著增多,同时Ⅱ组右侧新生微小血管数量与左侧相比明显增加(P<?0.01)。电镜观察可见Ⅱ组脑组织的细胞核、线粒体、内质网、高尔基体等超微结构均有缺血缺氧等病理改变。结论??成功构建了一种较新的兔单侧颈总动脉粥样硬化性闭塞模型,可通过该模型进行颅外段颈总动脉闭塞过程中血流动力学改变与脑组织超微结构改变?的相关研究。

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