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松江鲈(Trachidermusfasciatus)凝血因子XI的基因克隆与表达分析

DOI: 10.13560/j.cnki.biotech.bull.1985.2015.04.029, PP. 199-206

Keywords: 松江鲈,凝血因子XI,基因克隆,RealtimePCR

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Abstract:

克隆松江鲈凝血因子XI基因cDNA序列,分析表达模式。利用RACE技术从松江鲈中克隆获得了凝血因子XI的cDNA全长序列(命名为TfXI),并对其进行生物信息学和表达模式分析。获得TfXIcDNA全长1287bp,包括13bp的5'端非编码区,1143bp的开放阅读框以及131bp的3'端非编码区。开放阅读框编码280个氨基酸的多肽链,预测的蛋白大小为42.9kD。N端含有由第22-105位氨基酸,112-196位氨基酸、205-279位氨基酸和289-369位氨基酸形成的4个串联排列的典型APPLE结构域。NCBIBlast结果显示TfXI与其他物种凝血因子XI的相似性为30%-63%,进化树分析显示,TfXI符合传统进化规律。RealtimePCR分析表明,经LPS刺激96h后,松江鲈脾脏TfXI表达量明显提高(P<0.01)。

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