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利用转基因烟草表达人胰高血糖素样肽1的研究

, PP. 139-143

Keywords: 烟草,转基因,人胰高血糖素样肽1,生物活性

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Abstract:

人胰高血糖素样肽1(hGLP1)是一种短肽激素,是近年来备受关注的治疗糖尿病最有前景的候选药物。在设计合成hGLP1基因并构建植物表达载体的基础上,通过农杆菌介导将hGLP1基因导入烟草基因组中,获得转化再生植株,经过PCR扩增和Southernblot分析,证实hGLP1基因已整合进入6个株系的烟草基因组中。GUS组织化学染色表明,与目的基因融合的报告基因在转基因烟草中获得表达。Westernblot检测表明,其中2个株系转基因烟草叶片中能够检测到hGLP1融合蛋白的表达。初步的动物试验表明该融合蛋白具有一定的降血糖生物活性。

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