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甘蔗SoNIN1基因结构及其内含子信息分析

DOI: 10.13560/j.cnki.biotech.bull.1985.2014.12.024, PP. 147-152

Keywords: 甘蔗,中性/碱性转化酶,外显子,内含子,信息分析

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Abstract:

根据前期克隆得到的SoNIN1基因的全长cDNA和部分启动子序列设计引物,应用PCR技术从甘蔗叶片gDNA中克隆SoNIN1基因,并利用生物信息学方法分析基因的结构。结果表明,SoNIN1基因的DNA序列长4938bp,包含6个外显子和5个内含子,GenBank登录号KF563902。在该基因5'非翻译区存在一个268bp内含子序列,同时5个内含子序列中含有与低温、干旱和激素等胁迫响应相关的作用元件,这些可能对SoNIN1基因转录表达起调控作用。依据SoNIN1蛋白聚类和外显子-内含子基因结构可以将植物NINs分为两类。

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