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玉米弯孢叶斑病菌REMI白化突变体的筛选与致病性测定

DOI: 10.13560/j.cnki.biotech.bull.1985.2014.12.018, PP. 111-116

Keywords: 玉米弯孢菌叶斑病菌,REMI,白化菌株,致病性

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Abstract:

旨在探讨玉米弯孢叶斑病菌致病机制及遗传变异特性,利用限制性内切酶介导的基因整合技术(REMI)对Curvularialunata进行了遗传转化,共获得109个稳定的突变株。PCR检测结果表明,质粒pUC-JS已成功导入突变株中,转化子既有单拷贝也有多拷贝,白化菌株单拷贝插入频率为40%。此外,白化玉米弯孢叶斑病菌REMI突变株菌落颜色与野生菌株差异明显,并且在致病性上也明显弱于野生菌株;质粒拯救、测序与blast分析推测插入可能导致FAD3基因功能丧失。

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