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黄萎病菌诱导海岛棉酵母双杂交cDNA文库构建及评价

DOI: 10.13560/j.cnki.biotech.bull.1985.2014.12.017, PP. 105-110

Keywords: 黄萎病菌,海岛棉,酵母双杂交,cDNA文库

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Abstract:

采用SMARTIMcDNA合成技术,通过同源重组方法构建了海岛棉Pima90-53经强致病力黄萎病菌诱导的酵母双杂交cDNA文库。经测定,文库容量为2.82×106,滴度为5.02×108cfu/mL。菌落PCR显示,重组到pGADT7-Rec载体上的cDNA片段大小集中在500-1500bp之间,重组率93.33%。该文库完全可用于下一步互作蛋白筛选、信号转导组件确定及抗病蛋白结构和功能分析。

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