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病毒诱导的基因沉默及其在植物研究中的应用

DOI: 10.13560/j.cnki.biotech.bull.1985.2015.10.018, PP. 105-110

Keywords: 病毒诱导的基因沉默,基因功能鉴定

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Abstract:

病毒诱导的基因沉默(Virus-inducedgenesilencing,VIGS)在植物的基因功能鉴定中是一个非常有用的工具,在植物中了得到广泛应用。与传统的研究植物基因阻断的方法相比,VIGS具有简便高效、周期性短、不需要对植物进行转化以及可以沉默基因家族等特点,而且在一定条件下其沉默效应还能够传递给后代。介绍了VIGS的原理与操作过程,综述了该技术的应用与研究成果,有助于推动其在植物尤其是农作物育种中的进一步应用与发展。

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