Rapoport SI, Rao JS, Igarashim M. Brain metabolism of nutritionally essential polyunsaturated fatty acids depends on both the diet and the liver[J]. Prostaglandins Leukot Essent Fatty Acids, 2007, 77(5-6)：251-261.
[2]
Kuriki K, Hirose K, Wakai K, et al. Breast cancer risk and erythrocyte compositions of n-3 highly unsaturated fatty acids in Japanese[J]. Int J Cancer, 2007, 121(2)：377-385.
[3]
Cook HW. Fatty acid desaturation and chain elongation in eukaryote[M]// In Vance DE, Vance JE. Biochemistry of Lipids, Lipoproteins and Membranchs：Netherlands Elisevier, Amsterdam, 1996.
[4]
González-Rovira A, Mourente G, Zheng XZ, et al. ？Molecular and functional characterization and expression analysis of a Δ6 fatty acyl desaturase cDNA of European Sea Bass(Dicentrarchus labrax？L.)[J]. Aquaculture, 2009, 298(1-2)：90-100.
[5]
Li SL, Mai KS, Xu W, ？et al. Characterization, mRNA expression and regulation of Δ6 fatty acyl desaturase(FADS2)by dietary n-3 long chain polyunsaturated fatty acid(LC-PUFA)levels in grouper larvae(Epinephelus coioides)[J]. Aquaculture, 2014, 434(20)：212-219.
[6]
Owen JM, Adron JW, Middeton C, et al. Elongation and desaturation of dietary fatty acids in turbot Scophthalmus maximus L. and rainbow trout Salmo gairdnerii rich[J]. Lipids, 1975, 10(9)：528-531.
[7]
Zheng X, Seiliez I, Hasting N, et al. Characterization and comparison of fatty acyl Delta6 desaturase cDNAs from freshwater and marine teleost fish species[J]. Comp Biochem Physiol B Biochem Mol Biol, 2004, 139(2)：269-279.
Takeuchi T, Nakamoto Y, Hamasaki K, et al. Requirement of n-3 highly unsaturated fatty acids for larval swimming crab Portunus trituberculatus[J]. Nippon Suisan Gakkaishi, 1999, 65(5)：797-803.
[10]
Hashimoto K, Yoshizawa AC, Okuda S, et al. The repertoire of desaturases and elongases reveals fatty acid variations in 56 eukaryotic genomes[J]. J Lipid Res, 2008, 49(1)：183-191.
[11]
Seiliez I, Panserat S, Kaushik S, et al. Cloning, tissue distribution and nutritional regulation of a Delta6-desaturase-like enzyme in rainbow trout[J]. Comp Biochem Physiol B Biochem Mol Biol, 2001, 130(1)：83-93.
[12]
Zheng X, King Z, Xu Y, et al. Physiological roles of fatty acyl desaturases and elongases in marine fish：Characterisation of cNDAs of fatty acyl △6 desaturase and elovl5 elongase of cobia(Rachycentron canadum)[J]. Aquaculture, 2009, 290(1-2)：122-131.
[13]
Bell MV, Dick JR, Porter AE. Pyloric ceca are significant sites of newly synthesized 22：6n-3 in rainbow trout(Oncorhynchus mykiss)[J]. Lipids, 2003, 38(1)：39-44.
[14]
Morais S, Monroig O, Zheng XZ, et al. Highly unsaturated fatty acid synthesis in Atlantic salmon：characterization of ELOVL5- and ELOVL2-like elongases[J]. Mar Biotechnol, 2009, 11(1)：627-639.
[15]
Rasmussen BM, Vessby B, Uusitupa M, et al. Effects of dietary saturated, monounsaturated, and n-3 fatty acids on blood pressure in healthy subjects[J]. Am J Clin Nutr, 2006, 83(2)：221-226.
Sprecher H. Metabolism of highly unsaturated n-3 and n-6 fatty acids[J]. Biochi Biochys Acta, 2000, 1486(2-3)：219-231.
[18]
De Antueno RJ, Knickle LC, Smith H, et al. Activity of human Delta5 and Delta6 desaturases on multiple？n-3 and？n-6 polyunsaturated fatty acids[J]. FEBS Lett, 2001, 509(1)：77-80.
[19]
D’Andrea S, Guillou H, Jan S, et al. The same rat Delta6-desaturase not only acts on 18- but also on 24-carbon fatty acids in very-long-chain polyunsaturated fatty acid biosynthesis[J]. Biochem J, 2002, 364(part 1)：49-55.
[20]
Hastings N, Agaba M, Tocher DR, et al. A vertebrate fatty acid desaturase with Delta 5 and Delta 6 activities[J]. Proce Nation Acad Sci USA, 2001, 98(25)：14304-14309.
[21]
Zheng XZ, Tocher DR, Dickson CA. Highly unsaturated fatty acid synthesis in vertebrates：new innsights with the cloning and characterization of a △6-desaturase of Atlantic salmon[J]. Lipids, 2005, 40(1)：13-24.
[22]
Yang ZG, Guo ZH, Ji LY, et al. Cloning and tissue distribution of a fatty acyl △6-desaturase-like geneand effects of dietary lipid levels on its expression in the hepatopancreas of Chinese mitten crab(Eriocheir sinensis)[J]. Comparative Biochemistry and Physiology, 2013, 165(Part B)：99-105.
[23]
Wu X, Cheng Y, Sui L, et al. Effect of dietary supplementation of phospholipids and highly unsaturated fatty acids on reproductive performance and offspring quality of Chinese mitten crab, Eriocheir sinensis(H. Milne-Edwards), female broodstock[J]. Aquaculture, 2007, 273(4)：602-613.
Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T))method[J]. Methods, 2001, 25(4)：402-408.
[26]
Monroig O, Zheng X, Morais S, et al. Multiple genes for functional 6 fatty acyl desaturases(Fad)in Atlantic salmon(Salmo salar L.)：Gene and cDNA characterization, functional expression, tissue distribution and nutritional regulation[J]. Biochim Biophys Acta, 2010, 1801(9)：1072-1081.
