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黄芩醇提物对副溶血性弧菌抑制机制的研究

DOI: 10.13560/j.cnki.biotech.bull.1985.2015.08.023, PP. 159-165

Keywords: 黄芩,副溶血性弧菌,醇提物,抑菌机理

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Abstract:

从4种常见中药中筛选出对副溶血性弧菌有较好抑制作用的中药,并对其抑制机制进行研究。采用牛津杯法及液体倍比稀释法研究了中药醇提取物对副溶血性弧菌的敏感性;通过生长曲线和杀菌曲线、电镜观察以及SDS-PAGE等方法探讨黄芩醇提物的抑菌作用及机理。结果显示,4种中药醇提物对副溶血性弧菌表现出不同的抑菌活性,其中黄芩醇提物对副溶血性弧菌的抑制作用最强,抑菌圈为22.00±1.00mm,MIC和MBC均为3.91mg/mL。经MIC浓度处理后,细菌经过较长一段时间的延迟期,也能进入正常的对数期和稳定期;菌体表面变粗糙,出现裂解,菌液的蛋白含量明显升高,但是菌体的可溶性蛋白变化较小。结果表明,黄芩醇提物对副溶血性弧菌有很强的抑制效果,其作用是破坏细胞膜的完整性导致菌体裂解死亡。

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