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拟南芥GCR2参与感应N-丁酰基高丝氨酸内酯过程的初步研究

DOI: 10.13560/j.cnki.biotech.bull.1985.2015.08.014, PP. 94-101

Keywords: 群体感应,N-酰基高丝氨酸内酯,拟南芥,微量热泳动

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Abstract:

N-丁酰基高丝氨酸内酯(C4-HSL)是革兰氏阴性菌主要的群体感应信号,其能促进植物生长发育,激活细胞膜表面的Ca2+通道,从而参与调控植物的生理代谢。然而,植物感应C4-HSL的分子机制并不清楚。拟南芥GCR2作为ABA的受体,对植物的生理代谢十分重要。旨在探寻GCR2是否参与拟南芥感应C4-HSL的过程。qRT-PCR结果表明,C4-HSL处理后1h,GCR2基因表达量出现明显上调并在6h后达到最大值,说明C4-HSL可调节GCR2。ELISA结果显示,GCR2蛋白表达量也在6h达到最大值。对体外表达的GCR2进行纯化和浓缩,使其达到0.6mg/mL后进行微量热泳动(MST)检测。MST测得C4-HSL与GCR2的解离常数(Kd)为166nmol/L,显示出较强的结合能力。用BSA作为阴性对照,表明C4-HSL与GCR2的结合具有一定的特异性。这些结果表明GCR2可能参与了拟南芥感应C4-HSL的过程。

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