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RNAi技术及其在真菌基因功能研究中的应用

DOI: 10.13560/j.cnki.biotech.bull.1985.2015.08.008, PP. 50-58

Keywords: RNA干扰,基因沉默,真菌,基因功能

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Abstract:

RNA干扰是指在进化过程中由高度保守的外源或内源双链RNA(dsRNA)引发的转录后水平基因沉默的现象。RNAi技术作为一种高效、特异的基因阻断技术,已被广泛用于探索基因功能和传染性疾病及恶性肿瘤的治疗领域,成为2l世纪的研究热点之一。简要综述了RNAi的发现过程,作用机制,作用特点及其在真菌基因功能研究中的应用前景,为相关研究奠定理论基础。

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