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诱导烟草悬浮细胞氧爆发的海绵共附生真菌HMP-F66的筛选与鉴定

DOI: 10.13560/j.cnki.biotech.bull.1985.2015.07.026, PP. 174-179

Keywords: 米曲霉,黄曲霉,繁茂膜海绵,氧爆发,诱导抗性

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Abstract:

激发植物本身的天然免疫系统可作为防控植物病害的新途径。以50株分离自繁茂膜海绵的真菌为受试菌株筛选具有诱导植物抗性活性的菌株及其代谢产物。采用基于H2DCF-DA的荧光酶标法考察这些真菌的粗代谢物诱导烟草悬浮细胞产生氧爆发的活性,最终筛选到一株真菌HMP-F66能够显著诱导烟草悬浮细胞产生活性氧。根据其形态学特征、培养特征、特征性生理生化反应以及基于rDNAITS的分子生物学分析,推测该菌株为米曲霉(Aspergillusoryzae)。

References

[1]  Bennett JW. An overview of the genus Aspergillus[M]//. Machida M, Gomi K. Aspergillus:Molecular Biology and Genomics. Norfolk:Caiser Academic Press, 2010:1-17.
[2]  Hu X, Xia QW, Zhao YY, et al. Speradines F-H, Three new oxindole alkaloids from the marine-derived fungus Aspergillus oryzae[J]. Chemical and Pharmaceutical Bulletin, 2014, 62(9):942-946.
[3]  Felix G, Regenass M, Boller T. Specific perception of subnanomolar concentrations of chitin fragments by tomato cells:induction of extracellular alkalinization, changes in protein phosphorylation, and establishment of a refractory state[J]. The Plant Journal, 1993, 4(2):307-316.
[4]  Apel K, Hirt H. REACTIVE OXYGEN SPECIES:metabolism, oxidative stress, and signal transduction[J]. Annual Review of Plant Biology, 2004, 55(1):373-399.
[5]  Grant M, Lamb C. Systemic immunity[J]. Current Opinion in Plant Biology, 2006, 9(4):414-420.
[6]  Hammond-Kosack K, Jones JD. Plant Environment and Agriculture:Responses to plant pathogens[M]//. Buchanan B, Gruissem W, Jones RL. Biochemistry and Molecular Biology of Plants. Jonh Wiley & Sons, 2000:1102-1156.
[7]  齐祖同, 孔华忠, 孙曾美. 曲霉属及其相关有性型[M]//. 齐祖同. 中国真菌志. 北京:科学出版社, 1997:76-91.
[8]  Christensen M. A synoptic key and evaluation of species in the Aspergillus flavus group[J]. Mycologia, 1981, 73(6):1056-1084.
[9]  Machida M, Asai K, Sano M, et al. Genome sequencing and analysis of Aspergillus oryzae[J]. Nature, 2005, 438(7071):1157-1161.
[10]  Chang PK, Ehrlich KC. What does genetic diversity of Aspergillus flavus tell us about Aspergillus oryzae?[J]. International Journal of Food Microbiology, 2010, 138(3):189-199.
[11]  Chen Z, Agnew JL, Cohen JD, et al. Pseudomonas syringae type III effector AvrRpt2 alters Arabidopsis thaliana auxin physiology[J]. Proceedings of the National Academy of Sciences, 2007, 104(50):20131-20136.
[12]  Navarro L, Dunoyer P, Jay F, et al. A plant miRNA contributes to antibacterial resistance by repressing auxin signaling[J]. Science, 2006, 312(5772):436-439.
[13]  Samson R. Current Systematics of the Genus Aspergillus[M]//. Powell, KA, Renwick A, Peberdy JF. The Genus Aspergillus from taxonomy and genetics to industrial application. New York:Plenum Press, 1994:261-276.
[14]  Pfefferle W, Anke H, Bross M, et al. Asperfuran, a novel antifungal metabolite from Aspergillus oryzae[J]. The Journal of antibiotics, 1990, 43(6):648-654.
[15]  Camejo D, Martí MC, Jiménez A, et al. Effect of oligogalacturonides on root length, extracellular alkalinization and O2--accumulation in alfalfa[J]. Journal of Plant Physiology, 2011, 168(6):566-575.
[16]  O''Brien JA, Daudi A, Butt VS, et al. Reactive oxygen species and their role in plant defence and cell wall metabolism[J]. Planta, 2012, 236(3):765-779.
[17]  Fobert PR, Despres C. Redox control of systemic acquired resistance[J]. Curr Opin Plant Biol, 2005, 8(4):378-382.
[18]  Chen X, Mou Y, Ling J, et al. Cyclic dipeptides produced by fungus Eupenicillium brefeldianum HMP-F96 induced extracellular alkalinization and H2O2 production in tobacco cell suspensions[J]. World Journal of Microbiology and Biotechnology, 31(1):247-253.
[19]  Gerber I, Dubery I. Fluorescence microplate assay for the detection of oxidative burst products in tobacco cell suspensions using 2'', 7''-dichlorofluorescein[J]. Methods in Cell Science, 2004, 25(3-4):115-122.
[20]  Murakami H. Classification of the Koji Mold[J]. The Journal of General and Applied Microbiology, 1971, 17(4):281-309.
[21]  Kurtzman CP, Smiley MJ, Robnett CJ, et al. DNA Relatedness among wild and domesticated species in the Aspergillus flavus group[J]. Mycologia, 1986, 78(6):955-959.
[22]  Amaike S, Keller NP. Aspergillus flavus[J]. Annual Review of Phytopathology, 2011, 49(1):107-133.
[23]  Geiser DM, Pitt JI, Taylor JW. Cryptic speciation and recombination in the aflatoxin-producing fungus Aspergillus flavus[J]. Proceedings of the National Academy of Sciences, 1998, 95(1):388-393.
[24]  J?rgensen TR. Identification and toxigenic potential of the industr-ially important fungi, Aspergillus oryzae and Aspergillus sojae[J]. Journal of Food Protection, 2007, 70(12):2916-2934.
[25]  王楠, 刘祎, 胡江春, 等. 一种环匹阿尼酸类化合物及其制备和应用:中国, 201110447701. 6[P]. 2013-07-03.
[26]  Bari R, Jones JG. Role of plant hormones in plant defence respon-ses[J]. Plant Molecular Biology, 2009, 69(4):473-488.

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