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一个显性卷叶突变体z2的遗传分析与精细定位

DOI: 10.13560/j.cnki.biotech.bull.1985.2015.06.014, PP. 100-105

Keywords: 水稻(OryzasativaL.),卷叶突变体,遗传分析,图位克隆

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Abstract:

叶片的适度卷曲对水稻理想株型育种具有重要意义。在水稻T-DNA插入突变体库中获得一个叶片外卷突变体z2,突变体表型出现在分蘖期;与野生型相比,农艺性状无明显差异,光合作用效率高于野生型。对突变体和野生型的石蜡切片研究表明,突变体叶片泡状细胞数量(8-9个)明显多于野生型(4-5个)。z2卷叶性状遗传稳定,由一对显性核基因控制。以z2纯合突变体为母本,籼稻Dular为父本进行杂交构建F2代定位群体,利用图位克隆的方法,将该基因初定位于水稻第2号染色体的InDel1812与InDel1870标记之间,物理距离为580kb。

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