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三种DNA指纹图谱技术在菌株分型中的应用

DOI: 10.13560/j.cnki.biotech.bull.1985.2015.06.012, PP. 81-86

Keywords: 菌株分型,ERIC-PCR,BOX-PCR,RAPD,嗜热脂肪地芽孢杆菌

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Abstract:

旨在通过ERIC-PCR、BOX-PCR、RAPD等技术对4株地芽孢杆菌属菌株进行DNA指纹图谱分析,找到一种适合于地芽孢杆菌属尤其是嗜热脂肪地芽孢杆菌的菌株分型方法。4株地芽孢杆菌属菌株呈现出4种不同的指纹图谱,其中ERIC-PCR的方法获得的条带较为清晰,实验方法较为简便快捷,且能相对较好地区分不同株系;BOX-PCR的方法得到的条带相对较少,不能很好地区分同一种菌的不同株系;RAPD的方法获得的条带相对较多,区分性更高,但同时也增加了一部分工作量和成本。3种菌株分型技术均能有效的区分地芽孢杆菌属菌株,其中ERIC-PCR是一种最有效最便捷区分嗜热脂肪地芽孢杆菌不同株系的方法。

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