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北京鸭胚胎后肾间充质干细胞分离培养及鉴定

DOI: 10.13560/j.cnki.biotech.bull.1985.2015.06.028, PP. 177-182

Keywords: ,后肾间充质干细胞,生物学特性,诱导分化

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Abstract:

建立禽类后肾间充质干细胞(Metanephricmesenchymalstemcells,MMSCs)体外培养体系,研究其生物学特性和多向分化潜能。采用酶消化法分离北京鸭胚胎MMSCs,绘制生长曲线,通过免疫荧光和RT-PCR对MMSCs进行鉴定,诱导MMSCs向脂肪细胞和胰岛细胞分化。结果表明,鸭胚胎MMSCs具有良好的增殖活力,表达间充质干细胞(Mesenchymalstemcells,MSCs)特异性标志物,并诱导分化为脂肪细胞和胰岛细胞。北京鸭胚胎MMSCs在体外具有较强的自我更新能力及多向分化潜能,可作为组织工程的种子细胞进行保存。

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