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香蕉根际土壤解钾放线菌的筛选鉴定及解钾特性研究

DOI: 10.13560/j.cnki.biotech.bull.1985.2015.06.020, PP. 129-137

Keywords: 香蕉根际土壤,放线菌,解钾率

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Abstract:

旨在根据解钾菌的解钾特性和机理,从香蕉根际土壤中分离获得解钾能力较强且十分稳定的高效解钾放线菌。首先,通过解钾分离培养基和筛选培养基的初筛,获得一批高效解钾菌,然后在实验室条件下鉴定菌株类型和测定解钾率,得到一株高效解钾放线菌。最后通过形态观察、生理生化实验和16SrRNA基因序列分析对所筛菌株进行鉴定,并测定不同条件下的解钾率,对其解钾特性进行了研究。结果显示,从香蕉根际土壤中筛选出16株高效解钾菌,通过鉴定和解钾率的测定,最终获得一株稳定的解钾链霉菌(Streptomycete)M3-4,其在120h,30℃,pH为6,钾长石粉为5g,摇床转速为250r/min条件下,以麦芽糖为碳源,蛋白胨为氮源时解钾率达最大值。该菌株为陕西链霉菌(Streptomycesshaanxiensis),解钾率约为20%。

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