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烟草胞质6-磷酸葡萄糖酸脱氢酶基因的克隆及表达分析

DOI: 10.13560/j.cnki.biotech.bull.1985.2015.05.018, PP. 113-119

Keywords: 烟草,胞质6-磷酸葡萄糖酸脱氢酶,基因克隆,表达分析

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Abstract:

采用电子克隆的方法,结合RT-PCR和SMARTRACE技术,首次从烟草(Nicotianatabacum)中克隆到1个胞质6-磷酸葡萄糖酸脱氢酶(6PGDH)基因的cDNA序列,命名为Nt6PGDH(GenBank登录号:KM211534)。该基因cDNA全长1932bp,开放阅读框1455bp,编码484个氨基酸,与番茄(Solanumlycopersicum)和马铃薯(Solanumtuberosum)的6PGDH氨基酸序列一致性最高,为95%。生物信息学分析表明,Nt6PGDH氨基酸序列不存在信号肽和转运肽,无跨膜结构域,定位于细胞质。对烟草不同发育时期Nt6PGDH基因的表达情况分析发现,Nt6PGDH基因在烟草旺长期根、茎、叶中的表达量均高于苗期,并且在同一发育时期,烟草根中表达量最强,茎次之,叶片最弱。

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