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甲型H1N1流感病毒FAST-LAMP检测方法的建立

DOI: 10.13560/j.cnki.biotech.bull.1985.2015.05.013, PP. 78-83

Keywords: FAST-LAMP,甲型H1N1流感病毒,检测

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Abstract:

建立一种新型等温扩增检测方法,用于提高等温扩增反应的检测速度,应用于甲型H1N1流感病毒检测。根据HA基因设计一组引物,包括外引物、内引物、环引物及套内引物,套内引物的加入增加了引物与模板的接触位点,提高扩增效率,缩短检测时间。结果显示,FAST-LAMP检测法比普通LAMP检测法时间缩短45min左右,比加入环引物的LAMP检测方法缩短20min,大大缩短了反应的检测时间。检测灵敏度可达到1×102拷贝。FAST-LAMP是一种高效、更快的检测方法。

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