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亚硝化细菌原生质体化学诱变育种研究

, PP. 178-183

Keywords: 亚硝化细菌,原生质体,诱变,氯化锂,溴化乙锭

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Abstract:

以活性污泥中分离出的亚硝化细菌为研究对象,采用原生质体诱变技术,以化学诱变剂氯化锂、溴化乙锭诱变亚硝化细菌原生质体,选育亚硝酸盐氮富集能力高的菌株,同时研究了诱变剂浓度与脱氮能力之间的关系。结果表明,亚硝化细菌原生质体再生菌落的致死率随诱变剂浓度的增加而逐渐增大。采用1L模拟污水培养基扩大培养后,经2.5mg/mL氯化锂诱变的菌株LC002脱氮率为86.89%;经2μg/mL溴化乙锭诱变的菌株EB003脱氮率为85.67%。两株诱变菌株亚硝态氮富集能力与未诱变菌株相比有明显的提高。原生质体诱变技术是一种选育优良亚硝化细菌的有效方式。

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