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Ig启动子有效启动肿瘤抑制基因的表达研究

, PP. 136-141

Keywords: 肿瘤基因治疗,免疫球蛋白启动子,表达载体

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Abstract:

免疫球蛋白(Ig)启动子在多种上皮性肿瘤细胞具有高启动活性。采用PCR方法获得了Ig重链VH6-1启动子,用该启动子DNA序列替换真核表达载体(PDC316)中巨细胞白血病病毒启动子(CMV)序列,构建了Ig启动子/人增殖抑制基因(hHSG)/PDC316表达载体。结果显示,与CMV启动子相比,Ig启动子能够更有效地启动hHSG基因的表达,并显示其生长抑制作用和诱导凋亡作用。提示Ig启动子可作为肿瘤靶向治疗载体中的启动子,在肿瘤基因治疗研究中具有较好的应用前景。

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