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海门山羊MDFI基因SNP及其与体尺性状的关联分析

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Abstract:

采用PCR-SSCP和DNA测序技术,检测了18月龄海门母山羊共116只个体的MDFI基因所有外显子和部分内含子的遗传变异,分析了其遗传多态性和遗传结构,并对该基因的多态位点与海门山羊的体尺性状进行了相关分析。结果表明,在海门山羊MDFI基因的外显子3和外显子4共发现了2个SNPs,引起了1个无义突变和1个错义突变。与体尺性状的相关性分析发现,MDFI基因的2个SNPs与海门山羊的体高、胸围和管围有显著相关(P<0.05),可作为海门山羊分子育种的辅助标记。

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