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化学诱导后白木香转录组文库的构建与测序

, PP. 63-67

Keywords: 白木香,化学诱导,转录组文库,测序,组装,Unigenes

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Abstract:

采用改良异硫氰酸胍-CTAB法对5年生白木香树干经化学诱导后1年的各部分组织进行总RNA提取,提取到的总RNA经富集mRNA、打断、构建测序用cDNA文库后用于转录组测序,测序质量较高,Q20高达97.45%,共获得54685634条Cleanreads,总测序长度达4921707060nt,经初步组装,获得190109条Contigs序列,进一步组装,获得83467条Unigenes序列,总长度为58569625nt,平均长度为702nt,N50值高达1120,大于等于3000nt的Unigenes有1691条,占总Unigenes的2.03%,组装质量较高,使白木香的转录组信息得到较好的保存,为进行白木香结香相关的表达谱分析奠定基础。

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