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绒山羊DBY基因的BAC筛选与序列分析

, PP. 89-93

Keywords: 绒山羊,DBY,BAC,序列分析

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Abstract:

绒山羊是我国一种独特的生物资源,是经过长期的自然选择和人工选育而成的目前世界上产绒量最高、绒纤维品质最好的品种,所以提高绒山羊的繁殖力,对经济的发展有着非常重要的意义。运用PCR技术,对绒山羊Y染色体上控制精子生成的DBY基因进行BAC文库筛选、克隆、鉴定、测序,得到240bp的DBY基因片段。与GenBank收录的其他物种进行序列比对,结果显示,与绵羊序列的同源性达96%,与坡鹿、水牛的同源性达93%;与猪鹿、瘤牛和牛的同源性分别达92%和91%;而与人和黑猩猩及小鼠的序列无同源性,共编码52个氨基酸。物种间NJ的聚类结果与其在系统分类学的地位相一致。

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