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苹果锈果类病毒属属级寡核苷酸芯片筛查方法的建立

, PP. 151-156

Keywords: 苹果锈果类病毒属,属级探针,寡核苷酸芯片

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Abstract:

苹果锈果类病毒属是重要的植物类病毒属,目前尚无有效的筛查方法。通过对该属类病毒的核苷酸序列进行分析筛选,设计了35条用于该属类病毒筛查的属级特异性探针并制备了寡核苷酸芯片。应用苹果锈果类病毒及柑橘矮化类病毒标准样品对该芯片进行验证,结果表明所建立的属级芯片可以获得有效的杂交信号,其灵敏度与RT-PCR电泳法相当。该芯片可用于苹果锈果类病毒属类病毒的筛查,为该属类病毒的检疫与防控提供技术支撑。

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