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重组酵母产人IFN-α2a与LL-37融合蛋白的工艺优化

, PP. 214-218

Keywords: 干扰素α2a,LL-37,工艺

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Abstract:

通过单因素试验探索重组酵母P.pastorisGS115LI产人LL-37基因与IFN-α2a融合蛋白的最佳条件。结果显示,重组酵母菌产蛋白酶的最佳表达条件是利用BMMY为诱导培养基,以接种量OD600=5.5、温度26℃、pH6.0、诱导剂(甲醇)为每隔24h添加1.0%、振荡速度为200r/min条件下连续诱导144h。在最佳培养条件下,发酵液中的LL-37最高抗菌活性达27.9mm。与初始的发酵条件相比,人LL-37基因与IFN-α2a融合蛋白的产量提高了32.29%。

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