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合成唾液酸乳糖重组大肠杆菌的构建

, PP. 201-206

Keywords: 唾液酸乳糖,大肠杆菌,代谢工程

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Abstract:

唾液酸寡糖具有抗感染、提高免疫力、促进双歧杆菌增殖等功能,对其微生物合成方法的研究具有重要的理论和应用价值。克隆和表达来源于Campylobacterjejuni的N-乙酰葡萄糖胺异构酶基因(neuC)、乙酰神经氨酸合成酶基因(neuB)、CMP-乙酰神经氨酸合成酶基因(neuA)和来源于Nesseriameningitidis的唾液酸转移酶基因(nst),利用表达载体pSTV29,在大肠杆菌EscherichiacoliJM109内构建合成唾液酸乳糖的代谢途径。在接种量2%、装液量50mL/250mL三角瓶、摇床转速180r/min、温度34℃的条件下发酵30h,并以10g/L乳糖为底物,利用HPLC检测到唾液酸乳糖的合成量为2.45g/L,为人乳唾液酸寡糖及其类似物的异体合成提供了新的思路。

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