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香蕉枯萎病菌全局性调控因子FocVeA基因的克隆及功能预测

, PP. 101-106

Keywords: 香蕉枯萎病菌,FocVeA基因,克隆,序列分析

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Abstract:

根据轮枝样镰刀菌(Fusariumverticillioides)和藤仓镰刀菌(F.fujikuroi)veA同源基因相关序列设计引物,利用PCR与RT-PCR技术分别克隆了香蕉枯萎病菌2个生理小种的FocVeA基因序列和开放阅读框,同时对该基因的编码蛋白进行序列特征、系统发育与结构域分析。结果表明,2个生理小种的FocVeA基因(分别命名为F1VeA和F4VeA)的DNA片段全长分别为1693bp和1690bp,开放阅读框分别为1599bp和1596bp,分别编码532个和531个氨基酸。F1VeA和F4VeA基因预测氨基酸序列相似性均为99%,与GenBank中公布的Fusariumspp.veA基因氨基酸序列均有78%-99%相似性。系统聚类分析显示,F1VeA和F4VeA与GenBank中已登录的轮枝样镰刀菌(F.verticillioides)和藤仓镰刀菌(F.fujikuroi)等veA同源蛋白高度同源。蛋白质保守域搜索表明,FocVeA具有veA蛋白的功能域,属于veA蛋白家族的一员,推测该基因可能参与调控F.oxysporumf.sp.cubense的生长、毒素合成及致病性等。

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