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甘肃酒泉等地区豆科植物根瘤菌的遗传多样性和系统发育分析

, PP. 188-195

Keywords: 根瘤菌,16SrDNAPCR-RFLP分析,16SrDNA全序列分析

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Abstract:

采用16SrDNAPCR-RFLP和16SrDNA全序列分析技术对采集自甘肃酒泉、嘉峪关等8个地区的豌豆、菜豆等几个不同豆科植物根瘤中分离的53株供试菌株和9株参比菌株进行了遗传和系统发育分析。16SrDNAPCR-RFLP结果表明,在77.5%的相似性水平上全部供试菌株聚成5个分支。分支I为Sinorhizhobium-Rhizobium分支,分支II为Agrorhizobium-Bradyrhizobium分支,分支III是未知供试菌组成的分支,分支IV为Mesorhizobium分支,分支V为2株未知供试菌组成的分支。在89.8%的相似性水平上,分支I包括2个亚分支:亚分支1由CNU1008等14株供试菌组成,与SinorhizobiummelilotiLISPA1002菌株聚在一起;亚分支2包括6株未知菌,与RhizobiumleguminosarumUSDA2370聚在一起。分支II包括3个亚分支:亚分支1包括CNU1041等4株菌,与2株AgrobacteriumtumefaciensIAM13129T和IAM13569T聚在一起;亚分支2包括5株菌,与BradyrhizobiumjaponicumUSDA6菌株聚在一起;亚分支3包括3株未知菌。选取各分支和亚分支的代表菌株进行16SrDNA测序,结果表明,两种分析方法在结果上具有较好的一致性。处于分支I的Sinorhizobium亚分支的菌株,与S.fredii和S.meliloti的相似性达到99%,该亚分支菌株的确切系统发育地位有待于DNA-DNA杂交来确定。处于分支I的Rhizobium亚分支的菌株,与R.giardinii的相似性达到99%,与R.etli的相似性为98%。同样,该亚分支的确切地位有待于DNA-DNA杂交来确定。处于分支IIAgrobaterium亚分支的菌株与A.rubi的相似性达到99%。处于分支IIBradyrhizobium亚分支的菌株,与B.liaoningense的相似性达到99%。

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