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三角褐指藻的诱变育种及产EPA的条件研究

, PP. 114-119

Keywords: 三角褐指藻,EMS诱变,EPA

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Abstract:

旨在获得不饱和脂肪酯EPA产量更高的藻种,利用0.6%EMS对三角褐指藻进行诱变。通过单细胞分离技术得到1株突变株EP1,其EPA产量比出发藻株提高了19.81%。结果显示,突变藻株产EPA的最适条件为:NaNO375mg/L,pH7.5,昼夜温度17-15℃,接种量为12%。最适条件下培养7d,其EPA产量可达26.77mg/g。传代试验表明,突变藻株具有较好的遗传稳定性。

References

[1]  Kang SM, Kim AD, Heo SJ, et al. Induction of apoptosis by diphlor-etho hydroxycarmalol isolated from brown alga, Ishige okamurae[J]. Journal of Functional Foods, 2012, 4:433-439.
[2]  Takashi K, Takahiko I. Polysaccharides and antioxidant properties of aqueous solutions obtained from macroalgal beachcasts in the Noto Peninsula. Ishikawa, Japanese[J]. Food Chemistry, 2009, 112(3):575-581.
[3]  Posten C, Schaub G. Microalgae and terrestrial biomass as source for fuels:a process view[J]. Journal of Biotechnolog, 2009, 142:64-69.
[4]  扬世杰, 王希善, 李继芬, 等.螺旋藻培养初探[J]. 北京农业大学学报, 1998, 14(1):71-74.
[5]  王妮, 王素英, 师德强.螺旋藻诱变育种研究进展[J].食品与研究开发, 2009, 30(2):139-141.
[6]  降云峰, 刘永忠, 李万星, 等.甲基磺酸乙酯诱变技术在大豆育种上的应用[J].园艺与种苗, 2012, 11(6):12-15.
[7]  Bird R, Mck M, Neuffer G. Induced mutations in maize[M]// Janick J. Plant Breeding Reviews. New York:Van Nostrand Reinhold, 1987, 16(5):139-180.
[8]  Yongmanitchal W, Ward OP. Growth and production of omega-3 fatty acid by Phaeodactylum tricornulum under culture conditions[J]. Apply Environ Microbiology, 1991, 57(2):419-425.
[9]  蒋汉明, 张凤珍, 翟静, 等. ω-3多不饱和脂肪酸与人类健康[J].预防医学论坛, 2005, 11(1):65-69.
[10]  陈百灵.磷硅对三角褐指藻生长和脂肪酸组成的影响[D].青岛:中国海洋大学, 2011:55-56.
[11]  Elsey D, Jameson D, Raleigh B, et al. Fluoreseent measurement of microalgal neutral lipids[J]. J Microbiological Methods, 2007, 39(68):639-642.
[12]  Chen W, Zhang C, Song L. A high throughput Nile red method for quantitative measurement of neutral lipids in Microalgae[J]. Journal of Microbiological Methods, 2009, 37(77):41-47.
[13]  姚领, 胡萍, 胡蓓娟, 等.两种溶剂提取法提取三角褐指藻中不饱和脂肪酸的比较[J].食品工业科技, 2001, 5(12):114-116.
[14]  蔡阿根, 郑爱榕, 李文权, 等.海洋微藻中脂肪酸的气相色谱分析[J].海洋技术通报, 1998, 17(4):64-68.
[15]  Pahl SL, Lewis DM, Chen F, King KD. Heterotrophic growth and nutritional aspects of the diatom Cyclotella cryptica(Bacillariophyceae):Effect of some environmental factors[J]. Bioscience and Bioengineering, 2010, 109(3):235-239.
[16]  杨官品, 张继民, 魏东, 等.温度逆境处理提高拟微球藻EPA含量的研究[J].青岛海洋大学学报, 2002, 24(4):132-136.
[17]  赵爱娟.海洋微藻的诱变育种及其脂肪酸的测定[D].南京:南京理工大学, 2005:46-47.
[18]  Mata TM, Martins AA, Caetano NS. Microalgae for biodiesel production and other applications:a review[J]. Renew Sust Energy Rev, 2010, 14:217-232.
[19]  Go S, Lee SJ, Jeong GT. Factors affecting the growth and the oil accumulation of marine microalgae, Tetraselmis suecica[J]. Bioprocess Biosyst Eng, 2012, 35:145-150.
[20]  Widjaja A, Chien CC, Ju YH. Study of increasing lipid production from fresh water microalgae Chlorella vulgaris[J]. Journal of the Taiwan Institute of Chemical Engineers, 2009, 40(1):13-20.
[21]  Meng X, Yang J, Xu X, et al. Biodiesel production from oleaginous microorganisms[J]. Renewable Energy, 2009, 34(1):1-5.
[22]  钱振明, 邢荣莲, 汤宁, 等.光照和盐度对8种底栖硅藻生长及其生理生化成分的影响[J].烟台大学学报:自然科学与工程版, 2008, 21(1):46-52.

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