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一种乌鳢与斑鳢线粒体PCR-RFLP鉴定方法

, PP. 59-64

Keywords: 乌鳢,斑鳢,线粒体基因组,PCR-RFLP,鉴定

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Abstract:

为了高效的鉴别乌鳢与斑鳢,采用PCR-RFLP技术,对乌鳢与斑鳢开展分子生物学鉴定方法研究。通过比对乌鳢和斑鳢线粒体全序列,发现1处单核苷酸多态性位点,可以明确区分两个物种。利用1对引物对乌鳢与斑鳢线粒体基因组该区域进行PCR扩增,用限制性内切酶EcoRI分别对扩增产物进行酶切,并用1.5%的琼脂糖凝胶检测酶切结果。PCR-RFLP检测结果显示,斑鳢的PCR扩增产物被EcoRI酶切后生成两个不同大小的片段,分别为315bp和875bp,乌鳢则保持不变。由此可将乌鳢与斑鳢在酶切图谱上鉴别出来。

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