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食源性沙门氏菌耐药性及质粒介导喹诺酮耐药基因检测

, PP. 202-207

Keywords: 食源性,沙门氏菌,耐药性,质粒介导,喹诺酮类

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Abstract:

随机采集的638份食品样品中沙门氏菌总检出率为9.7%(n=62株),共检出16种不同的血清型,其中最常见的为鸭沙门氏菌。受试菌株对磺胺甲基异噁唑、复方新诺明、链霉素和环丙沙星的耐药率较高。16株耐环丙沙星沙门氏菌按GyrA和ParC喹诺酮耐药决定区(QRDR)不同氨基酸替代组合可分为5种突变型,其中GyrA亚基发生Ser83Phe和Asp87Gly变异,同时ParC亚基发生Ser80Arg变异为最常见的突变类型。62株食源性沙门氏菌中,qnr基因阳性的菌株共7株,占受试菌株的11.3%。qnrA和qnrS基因阳性菌株分别有2株和5株,没有菌株携带qnrB、qnrC和qnrD基因。aac(6')-Ib基因阳性菌株共有8株,其中3株经确认为携带其变体基因aac(6')-Ib-cr。结果表明,新乡市食源性沙门氏菌血清型分布呈多样性,耐药状况较为严重,并且一些菌株携带质粒介导喹诺酮耐药(PMQR)基因。

References

[1]  杨保伟.食源性沙门氏菌特性及耐药机制研究[D].杨凌:西北农林科技大学, 2010.
[2]  杨保伟, 曲东, 申进玲, 等.陕西食源性沙门氏菌耐药及相关基因[J].微生物学报, 2010, 50(6):788-796.
[3]  黄道超, 姜波, 杨光友, 等.凹甲陆龟沙门氏菌的分离鉴定及毒力因子基因的PCR检测[J].生物技术通报, 2009(10):156-160.
[4]  Deng X, Ran L, Wu S, et al. Laboratory-based surveillance of non-typhoidal Salmonella infections in Guangdong Province, China[J]. Foodborne Pathog Dis, 2012, 9(4):305-312.
[5]  张秀丽, 杨保伟, 廖兴广, 等.河南省鹤壁市食源性和禽源性沙门氏菌耐药性研究[J].中国人兽共患病学报, 2010, 26(12):1134-1136.
[6]  Barbosa TM, Levy SB. The impact of antibiotic use on resistance development and persistence[J]. Drug Resist Update, 2000, 3(5):303-311.
[7]  Veldman K, Cavaco LM, Mevius D, et al. International collaborative study on the occurrence of plasmid-mediated quinolone resistance in Salmonella enteric and Escherichia coli isolated from animals, humans, food and the environment in 13 European countries[J]. J Antimicrob Chemother, 2011, 66(6):1278-1286.
[8]  只帅, 席美丽, 申进玲, 等.食源性大肠杆菌耐药性检测[J].西北农业学报, 2009, 18(6):377-381.
[9]  Jiang Y, Zhou Z, Qian Y, et al. Plasmid-mediated quinolone resistance determinants qnr and aac(6'')-Ib-cr in extended-spect-rum β-lactamase-producing Escherichia coli and Klebsiella pneumo-niae in China[J]. J Antimicrob Chemother, 2008, 61(5):1003-1006.
[10]  Wang M, Guo Q, Xu X, et al. New plasmid-mediated quinolone resistance gene, qnrC, found in a clinical isolate of Proteus mirabilis[J]. Antimicrob Agents Chemother, 2009, 53(5):1892-1897.
[11]  Cavaco LM, Hasman H, Xia S, et al. qnrD, a novel gene conferring transferable quinolone resistance in Salmonella enterica serovar Kentucky and Bovismorbificans strains of human origin[J]. Antimicrob Agents Chemother, 2009, 53(2):603-608.
[12]  关文英, 申志新, 张淑红, 等.河北省食品中沙门氏菌的耐药性研究[J].现代预防医学, 2006, 33(10):1761-1763.
[13]  Yang B, Qiao L, Zhang X, et al. Serotyping, antimicrobial susceptibility, pulse field gel electrophoresis analysis of Salmonella isolates from retail foods in Henan Province, China[J]. Food Control, 2013, 32(1):228-235.
[14]  Yue L, Jiang HX, Liao XP, et al. Prevalence of plasmid-mediated quinolone resistance qnr genes in poultry and swine clinical isolates of Escherichia coli[J]. Vet Microbiol, 2008, 132(3):414-420.
[15]  Jiang H, Tang D, Liu Y, et al. Prevalence and characteristics of β-lactamase and plasmid-mediated quinolone resistance genes in Escherichia coli isolated from farmed fish in China[J]. J Antimicrob Chemother, 2012, 67(10):2350-2353.
[16]  Robicsek A, Strahilevitz J, Jacoby GA, et al. Fluoroquinolone-modifying enzyme:a new adaptation of a common aminoglycoside acetyltransferase[J]. Nat Med, 2006, 12:83-88. ?

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