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一株产阿魏酸酯酶丝状真菌的分离鉴定及其粗酶性质研究

, PP. 162-167

Keywords: 阿魏酸酯酶,菌种鉴定,互隔交链格孢霉,酶学性质

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Abstract:

利用平板透明圈法,筛选分离到37株具有阿魏酸酯酶活性的丝状真菌,其中1株编号为HA4087的菌株具有较强的产阿魏酸酯酶能力。经形态学观察、18SrDNA和ITS序列鉴定为互隔交链格孢霉。对该菌株所产阿魏酸酯酶的粗酶性质进行了初步研究,结果表明,在发酵培养基中发酵粗酶活力为86mU/mL,最适作用温度为55℃,最适pH值为5.5;在55℃保温30min后酶活力仍为90%以上;在pH4.5-6.5范围内稳定性较好,酶活仍为85%以上。试验获得了产高酶活阿魏酸酯酶的菌株,为阿魏酸酯酶的工业化应用提供前提。

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