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OsPT8过量表达提高转基因烟草的耐低磷能力

, PP. 106-111

Keywords: 烟草,耐低磷,高亲和磷转运蛋白,OsPT8

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Abstract:

高亲和磷转运蛋白负责植物在低磷条件下吸收和转运磷酸盐,对植物的生长发育至关重要。将水稻中关键的高亲和磷转运蛋白基因OsPT8(AhighaffinityphosphatetransportergeneOsPht1;8,以下简称OsPT8)通过农杆菌介导的方法转入烟草云烟87,以转基因烟草和野生型(云烟87)为材料,设置正常供磷(1mmol/LPi)和低磷(0.1mmol/LPi)两个处理的沙培试验,检测烟株地上部和地下部的生物量、全磷及有效磷的含量,分析烟草高亲和磷转运蛋白家族基因(NtPT1和NtPT2)的表达差异。结果显示,低磷条件下,OsPT8过量表达转基因株系生物量均显著高于野生型;在正常供磷和低磷条件下,OsPT8过量表达烟草株系全磷含量和有效磷含量均显著高于野生型,这表明高亲和磷转运蛋白基因OsPT8可以提高转基因烟草的耐低磷能力。RT-PCR和Q-PCR结果显示,转基因株系显著提高了烟草高亲和磷转运蛋白基因NtPT1和NtPT2的表达量,表明OsPT8对烟草磷吸收和转运的影响是通过OsPT8基因和烟草NtPT1、NtPT2基因等一个复杂的过程起作用的。

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