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拟南芥原生质体瞬时表达快速验证重组酶外源基因删除

, PP. 86-92

Keywords: 位点特异性重组系统,热激蛋白Hsp18.2启动子,拟南芥原生质体瞬时表达

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Abstract:

通过在拟南芥原生质体中瞬时表达重组酶删除系统,以拟南芥热激蛋白Hsp18.2基因的启动子诱导加入马铃薯StLs1基因第2个内含子的重组酶表达,证明瞬时转化质粒DNA中位于识别位点之间的基因元件可以被有效删除。建立了一种快速验证重组酶外源基因删除系统的方法。

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