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嗜热绿色糖单孢菌木聚糖酶XyN10A基因在毕赤酵母中的分泌表达及其酶学性质研究

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Keywords: 绿色糖单孢菌,木聚糖酶,分泌表达,毕赤酵母

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Abstract:

木聚糖酶(Xylanase)可降解自然界中大量存在的木聚糖类半纤维素,由于其在饲料、食品、造纸等领域的广泛应用,越来越受到人们的关注。利用分子生物学技术首次成功构建了一株高效表达嗜热绿色糖单孢菌(Saccharomonosporaviridis)木聚糖酶SviXyN10A的重组工程菌,建立了SviXyN10A的毕赤酵母表达体系,实现了SviXyN10A在毕赤酵母(Pichiapastoris)中的分泌表达,并对表达产物进行了诱导表达及纯化,以及酶学性质的研究。结果表明,重组木聚糖酶SviXyn10A用甲醇诱导2d后酶活即达到7.53IU/mL,最适反应温度为70℃,pH7.0。pH作用范围较广(pH5.0-9.0),具有较强的热稳定性和耐碱性,几乎没有纤维素酶活性,故可以应用在很多工业领域,尤其是在纸浆造纸行业具有很大的潜在应用价值。

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