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质谱非标记定量分析小麦抗条锈病近等基因系蛋白质变化

, PP. 88-95

Keywords: 小麦,条锈病,抗病性,蛋白质组学,非标记定量Q,Exactive质谱

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Abstract:

利用基于液相色谱串联Orbitrap质谱(QExactive)的非标定量(Label-free)技术,对小麦抗病单基因近等基因系Taichung29*6/Yr10和背景品种Taichung29叶片的蛋白质表达进行了比较分析。经MASCOT软件搜库,在两个样品中共同鉴定到2257个蛋白,其中有准确定量信息的蛋白共1549个,含量变化大于2倍的差异蛋白有102个。对102个差异表达蛋白进行了GeneOntology(GO)功能注释和分析,发现他们多数定位于细胞基质和核糖体等细胞器,具备结合、催化等功能,主要参与代谢、细胞过程、应激等生物学进程,其中超氧化物歧化酶、甲硫氨酰氨肽酶、溶酶体-β-葡萄糖苷酶和铁蛋白等可能与小麦抗病单基因近等基因系Taichung29*6/Yr10的抗病性有一定相关性。

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