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敲减FST基因对猪成纤维细胞中相关基因表达的影响

, PP. 111-114

Keywords: 卵泡抑素(FST),RNA干扰,猪胎儿成纤维细胞,相关基因

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Abstract:

旨在获得敲减FST基因的猪胎儿成纤维细胞,并探讨对相关基因表达的影响。将构建成功并鉴定准确的发夹RNA真核表达载体FR1-shRNA利用脂质体转染至猪胎儿成纤维细胞中,并进行G418筛选,获得稳定转染细胞株,并利用Real-time检测了相关基因表达的变化。结果表明,在稳定转染的细胞株中,FST基因的表达受到显著抑制,结果导致了ActivinRⅡA、Myostatin和Bmp4基因的表达出现下调趋势,并且极显著地降低MyoD基因的表达。

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