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6株酵母菌产营养活性物质能力的比较分析

DOI: 10.3969/j.issn.1006-267x.2014.02.032

Keywords: 酵母菌,营养活性物质,反刍动物微生态制剂

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Abstract:

本试验旨在为后期反刍动物微生态制剂研制筛选出高活性酵母菌株。试验测定了6株酵母菌[产朊假丝酵母(Cadidaatilis)BY、热带假丝酵母(Candidatropicalis)BR、热带假丝酵母B2、酿酒酵母(Saccharomycescerevisiae)YR、酿酒酵母YC和酿酒酵母BC]产营养活性物质（β-葡聚糖、甘露聚糖、有机酸、氨基酸和多肽）的能力，并使用DPS14.10软件运用Topsis法对试验数据进行了多指标综合分析。结果表明：热带假丝酵母BR产β-葡聚糖、甘露聚糖能力最强，其细胞壁中β-葡聚糖、甘露聚糖含量分别为80.2、60.5mg/g；产朊假丝酵母BY产有机酸能力最强，其培养液中总有机酸含量为344.46μg/mL；酿酒酵母BC产多肽能力最强，其培养液中多肽含量为14.08mg/dL。多指标综合分析结果表明最优菌株为热带假丝酵母BR，其产β-葡聚糖、甘露聚糖、丁二酸、谷氨酸和半胱氨酸的能力均最强，其细胞壁中β-葡聚糖、甘露聚糖含量分别为80.2、60.5mg/g，培养液中总有机酸、总氨基酸含量分别达到312.11μg/mL、4.705mg/g。通过6株酵母菌产营养活性物质能力综合比较分析得出最优菌株为热带假丝酵母BR。

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