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湖羊小肠黏膜内参基因筛选

DOI: 10.3969/j.issn.1006-267x.2015.11.019

Keywords: 实时定量PCR,内参基因,湖羊,小肠黏膜

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Abstract:

为选择湖羊小肠黏膜合适实时定量PCR的内参基因,本试验选用6只湖羊公羔,分别在42和84日龄各屠宰3只,研究候选内参基因甘油醛-3-磷酸脱氢酶(GAPDH)、磷酸甘油酸激酶基因1(PGK1)、18SrRNA和β-肌动蛋白(ACTB)在十二指肠、空肠、回肠黏膜发育过程中表达是否稳定。结果表明:4个候选基因都获得特异性扩增片段;在不同的小肠肠段黏膜中,PGK1和ACTB表达最稳定,在不同日龄4个候选基因表达都较稳定;ΔCt法和geNorm软件分析表明,ACTB表达最稳定。综上,利用实时定量PCR研究湖羊小肠黏膜的内参基因最佳选择为ACTB。

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