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饲粮蛋白质水平对家兔食欲肽基因表达的影响

DOI: 10.3969/j.issn.1006-267x.2015.09.035

Keywords: 家兔,饲粮蛋白质,食欲肽

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Abstract:

本试验旨在探讨饲粮蛋白质水平对家兔食欲肽基因表达的影响。选择体重相近的30日龄断奶伊拉肉兔30只,随机分为3组,分别饲喂蛋白质水平为12.8%、16.0%和19.2%的试验饲粮,每组10个重复,每个重复1只兔。预试期7d,正试期35d。试验结束后,采集弓形核、十二指肠、空肠和回肠,测定弓形核中阿片促黑色素原(POMC)、可卡因-苯丙胺调节转录物(CART)、神经肽Y(NPY)、刺鼠基因相关蛋白(AgRP)、胆囊收缩素(CCK)和肠道中CCK基因的相对表达量。结果显示:十二指肠CCK基因的相对表达量显著高于空肠、回肠和弓形核(P0.05);与16.0%和12.8%蛋白质饲粮组相比,19.2%蛋白质饲粮组弓形核中POMC和CART基因的相对表达量显著增加(P0.05)。由此得出,饲喂低蛋白质水平(12.8%~16.0%)饲粮家兔的食欲增加与肠道和中枢中脑肠肽有关。与低蛋白质水平饲粮相比,高蛋白质水平(19.2%)饲粮显著提高十二指肠中CCK和弓形核中CART和POMC基因的相对表达量,表明这些基因形成食欲网络共同参与饲粮蛋白质调控家兔食欲的过程。此外,回肠和弓形核中CCK、弓形核中NPY和AgRP基因对饲粮中蛋白质水平变化不敏感。

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