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花鲈摄食调控相关因子全长cDNA的克隆和序列分析

DOI: 10.3969/j.issn.1006-267x.2015.09.014

Keywords: 基因克隆,摄食调控,花鲈,雷帕霉素靶蛋白,核糖体蛋白S6激酶beta-1,神经肽Y前体,脑肠肽前体

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Abstract:

本试验采用反转录聚合酶链式反应(RT-PCR)和cDNA末端快速扩增(RACE)技术克隆花鲈(Lateolabraxjaponicus)调控摄食因子雷帕霉素靶蛋白(mTOR)、核糖体蛋白S6激酶beta-1(S6K1)、神经肽Y前体(NPYprecursor)和脑肠肽前体(preproghrelin)cDNA全长序列。结果显示:花鲈mTOR(GenBank登录号KJ746670)cDNA全长8296bp,开放阅读框7551bp,编码2516个氨基酸,预测其分子质量为286.14ku,与其他物种的相似性为62.0%~98.0%;S6K1(GenBank登录号KJ746671)cDNA全长2232bp,开放阅读框1539bp,编码512个氨基酸,预测其分子质量为56.87ku,与其他物种的相似性为80.4%~84.9%;NPYprecursor(GenBank登录号KJ850326)cDNA全长676bp,开放阅读框300bp,编码99个氨基酸,预测其分子质量为11.26ku,与其他物种的相似性为53.6%~99.0%;preproghrelin(GenBank登录号KJ850327)cDNA全长1201bp,开放阅读框324bp,编码107个氨基酸,预测其分子质量为12.03ku,与其他物种的相似性为19.6%~85.0%。花鲈摄食调控因子cDNA全长序列地获得将为进一步研究其摄食调控机理奠定基础。

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