全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

山羊瘤胃与粪便微生物多样性

DOI: 10.3969/j.issn.1006-267x.2015.08.030

Keywords: 山羊,IlluminaMiSeq,瘤胃,粪便,微生物

Full-Text   Cite this paper   Add to My Lib

Abstract:

本试验旨在应用高通量测序技术比较山羊瘤胃和粪便微生物的结构与组成。选取6只10月龄波尔山羊,饲喂精粗比为3:7的饲粮14d后采集瘤胃液(R组)和粪便样品(F组)(每组6个重复,每个样品为1个重复)。提取总DNA后用古菌/细菌16SrRNA通用引物扩增V4~V5区,并用IlluminaMiSeq平台测序。结果表明:1)共获得有效序列227729条,聚类后得13601个运算分类单位(OTU)。2)所得OTU经物种注释99.337%被归类为细菌界,F组相对丰度最高优势菌门为厚壁菌门(65.400%),R组相对丰度最高优势菌门为拟杆菌门(60.188%)。3)从所有样品中共检测到129个科,F组中相对丰度最高为瘤胃球菌科(37.705%),极显著高于R组(P<0.01),而R组中,相对丰度最高的为普雷沃氏菌科(29.959%),极显著高于F组(P<0.01)。4)在粪便样品和瘤胃液样品种共检测到258个属,F组相对丰度最高的属为瘤胃球菌科未分类的属(26.914%),R组相对丰度最高为普雷沃菌属(28.621%)。5)所有12个样品间共发现了14个共享属,其中相对丰度最高的为厚壁菌门下的梭菌属4(Clostridium_Ⅳ,1.748%)。本试验结果表明瘤胃和粪便中微生物组成存在着较大差异,瘤胃中还有许多未被分类鉴定且相对丰度较高的微生物,需要进一步研究。

References

[1]  张丽英.饲料分析及饲料质量检测技术[M].北京:中国农业大学出版社,2003.
[2]  VAN DONKERSGOED J,GRAHAM T,GANNON V.The prevalence of verotoxins,Escherichia coli O157:H7,and Salmonella in the feces and rumen of cattle at processing[J].The Canadian Veterinary Journal,1999,40(5):332.
[3]  MIN B R,SOLAIMAN S,SHANGE R,et al.Gastrointestinal bacterial and methanogenic archaea diversity dynamics associated with condensed tannin-containing pine bark diet in goats using 16S rDNA amplicon pyrosequencing[J/OL].International Journal of Microbiology,2014.http://dx.doi.org/10.1155/2014/141909.
[4]  AVGUTIN G,WALLACE R J,FLINT H J.Phenotypic diversity among ruminal isolates of Prevotella ruminicola:proposal of Prevotella brevis sp.nov.,Prevotella bryantii sp.nov.,and Prevotella albensis sp.nov.and redefinition of Prevotella ruminicola[J].International Journal of Systematic Bacteriology,1997,47(2):284-288.
[5]  DOERNER K C,WHITE B A.Assessment of the endo-1,4-beta-glucanase components of Ruminococcus flavefaciens FD-1[J].Applied and Environmental Microbiology,1990,56(6):1844-1850.
[6]  GIRIJA D,DEEPA K,XAVIER F,et al.Analysis of cow dung microbiota-a metagenomic approach[J].Indian Journal of Biochemistry,2013,12:372-378.
[7]  DE JESùS-LABOY K M,GODOY-VITORINO F,PICENO Y M,et al.Comparison of the fecal microbiota in feral and domestic goats[J].Genes,2011,3(1):1-18.
[8]  FREY J C,PELL A N,Berthiaume R,et al.Comparative studies of microbial populations in the rumen,duodenum,ileum and faeces of lactating dairy cows[J].Journal of Applied Microbiology,2010,108(6):1982-1993.
[9]  冯仰廉.反刍动物营养学[M].北京:科学出版社,2004.
[10]  WILLIAMS A G,COLEMAN G S.The rumen protozoa[M].New York:Springer-Verlag; 1992.
[11]  MACKIE R I,WHITE B A.Recent advances in rumen microbial ecology and metabolism:potential impact on nutrient output[J].Journal of Dairy Science,1990,73(10):2971-2995.
[12]  HESPELL R B,AKIN D E,DEHORITY B A.Bacteria,fungi,and protozoa of the rumen[C]//MACKIE R I,WHITE B A,ISAACSON R E.Gastrointestinal microbiology.New York:Chapman and Hall,1997.
[13]  DE OLIVEIRA M N V,JEWELL K A,FREITAS F S,et al.Characterizing the microbiota across the gastrointestinal tract of a Brazilian Nelore steer[J].Veterinary Microbiology,2013,164(3):307-314.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133