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异丁酸对犊牛瘤胃及小肠黏膜基因表达的影响

DOI: 10.3969/j.issn.1006-267x.2015.08.021

Keywords: 异丁酸,犊牛,瘤胃黏膜,小肠黏膜,基因表达

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Abstract:

本试验旨在研究饲粮补充异丁酸对断奶前后犊牛瘤胃及小肠发育相关基因表达的影响。选取平均体重(40.0±0.2)kg、发育正常、30日龄的中国荷斯坦公犊牛36头,随机分为4组,对照组饲喂基础饲粮,试验组分别在基础饲粮的基础上添加异丁酸3、6和9g/d。分别于45、60(断奶)、90日龄每组屠宰3头并采集瘤胃及小肠黏膜组织样品,通过实时荧光定量PCR技术检测生长激素受体(GHR)、胰岛素受体(INSR)、3-羟基-3-甲基戊二酰辅酶A合成酶1(HMGCS1)和钠-葡萄糖共转运载体1(SGLT1)的mRNA表达量。结果表明:犊牛体重和GHR、INSR、HMGCS1mRNA的表达量均随着犊牛日龄的增长而出现显著增长(P<0.05),而SGLT1mRNA的表达量在断奶前后随着日龄的增长先下降后增长,变化显著(P<0.05)。45和60日龄时,犊牛体重在9g/d异丁酸组显著高于对照组(P<0.05),但与6g/d异丁酸组差异不显著(P>0.05)。各日龄6和9g/d异丁酸组瘤胃和小肠各部位GHR、INSR、HMGCS1mRNA的表达量均高于对照组,且这2组之间差异不显著(P>0.05)。添加异丁酸对45日龄和90日龄犊牛小肠各部位SGLT1mRNA的表达量没有显著影响(P>0.05);60日龄6和9g/d异丁酸组十二指肠SGLT1mRNA表达量显著高于对照组和3g/d异丁酸组(P<0.05),9g/d异丁酸组空肠近端、空肠远端和回肠SGLT1mRNA表达量显著高于对照组(P<0.05),但与6g/d异丁酸组差异不显著(P>0.05)。综合以上结果,饲粮中补充异丁酸能够促进犊牛生长和瘤胃及小肠黏膜的发育,本试验条件下其适宜添加量为6g/d。

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