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不同脂肪源饲粮育成猪肝脏转录组差异分析

DOI: 10.3969/j.issn.1006-267x.2015.07.018

Keywords: 红花籽油,椰子油,转录组,高通量RNA测序,差异表达基因,代谢通路,肝脏,育成猪

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Abstract:

本试验旨在肥育猪饲粮中分别添加红花籽油和椰子油,采集育成猪肝脏组织,进行高通量转录组测序,找出2种处理间的差异表达基因。利用IlluminaHiSeqTM2500高通量RNA-seq测序技术对2种处理育成猪肝脏进行转录组测序,使用TopHat2软件将测序得到的Reads序列与猪参考基因组(Sscrofa10.2)序列比对,找出差异表达基因,并在Nr、GO和KEGG数据库中进行功能注释、富集分析和聚类分析。结果显示:红花籽油组和椰子油组肝脏差异表达基因共有938个,与椰子油组相比,红花籽油组表达上调基因有479个,下调基因有459个;GO功能分类注释到细胞组成、生物学过程和分子功能数据库中差异表达基因数分别有773、768和729个;注释到KEGG通路中差异表达基因数346个,显著富集通路为酮体生成与降解通路和类萜骨架生物合成通路(P<0.05)。

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