全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

硝酸钠和2-溴乙烷磺酸钠对山羊体外瘤胃发酵甲烷、氢气和挥发性脂肪酸生成的影响

DOI: 10.3969/j.issn.1006-267x.2015.05.031

Keywords: 体外瘤胃发酵,发酵类型,甲烷减排,甲烷抑制剂,硝酸盐还原菌

Full-Text   Cite this paper   Add to My Lib

Abstract:

本试验从瘤胃氢代谢生物化学过程的角度出发,研究硝酸钠和2-溴乙烷磺酸钠(BES)对瘤胃甲烷生成的抑制机制。选用2只装有永久性瘤胃瘘管的成年湘东黑山羊作为瘤胃液供体,设对照组、硝酸钠组(添加剂量分别为0.10、0.20和0.40mg/mL)和BES组(添加剂量分别为0.63、1.26和2.52mg/L),采用全自动体外模拟瘤胃发酵设备,进行24h体外瘤胃发酵试验,测定产气量、氢气和甲烷的产量和含量、挥发性脂肪酸产量及组成。结果表明:与对照组相比,添加硝酸钠和BES后,甲烷产量显著降低(P<0.05),并且随添加剂量增加,呈现线性下降的变化趋势(P<0.05)。低剂量硝酸钠(≤0.20mg/mL)对产气量、起始底物降解速率、乙酸、丙酸产量和乙丙比无显著影响(P>0.05);高剂量硝酸钠(0.40mg/mL)显著提高了氢气产量及含量,显著降低了产气量、甲烷产量及含量、起始底物降解速率和总挥发性脂肪酸产量(P<0.05);添加BES对产气量、起始底物降解速率和总挥发性脂肪酸产量没有显著影响(P>0.05),但显著增加了氢气产量及含量(P<0.05),显著降低了乙丙比(P<0.05),发酵类型向丙酸型转变。由此可见,硝酸钠减少甲烷生成依靠其氢池和瘤胃微生物毒性作用,而BES减少甲烷生成依靠抑制甲烷产生,对气体生成和饲料的降解影响很小。

References

[1]  刘开朗,王加启,卜登攀,等.瘤胃甲烷调控方法评述[J].中国微生态学杂志,2009,21(4):354-358.
[2]  NOLLET L,DEMEYER D,VERSTRAETE W.Effect of 2-bromoethanesulfonic acid and peptostreptococcus productus ATCC 35244 addition on stimulation of reductive acetogenesis in the ruminal ecosystem by selective inhibition of methanogenesis[J].Applied and Environmental Microbiology,1997,63(1):194-200.
[3]  张春梅.植物油及十八碳不饱和脂肪酸对瘤胃甲烷生成和微生态的影响[D].博士学位论文.杭州:浙江大学,2008.
[4]  MUETZEL S,HUNT C L,TAVENDALE M H.Brief communication:evaluating rumen fluid from sheep and cattle as inoculum in a newly developed automated in vitro rumen batch culture system[J].Proceeding of the New Zealand Society of Animal Production,2011,71:240-242.
[5]  WANG M,TANG S X,TAN Z L.Modeling in vitro gas production kinetics:derivation of logistic-exponential (LE) equations and comparison of models[J].Animal Feed Science and Technology,2011,165(3/4):137-150.
[6]  NOLLT L,DEMEYER D,VERSTRAEE W.Effect of 2-bromoethanesulfonic acid and peptostreptococcus productus ATCC 35244 addition on stimulation of reductive acetogenesis in the ruminal ecosystem by selective inhibition of methanogenesis[J].Applied and Environment Microbiology,1997,63(1):194-200.
[7]  TOMKINS N W,HUNTER R A.Methane mitigation in beef cattle using a patented antimethanogen[C]//ECKARD R.Proceedings of the 2nd Joint Australia and New Zealand Forum on non-CO2 greenhouse gas emissions from agriculture.Canberra:Cooperative Research Centre for Greenhouse Accounting,2003.
[8]  IWAMOTO M,ASANMA N,HINO T.Effects of pH and electron donors on nitrate and nitrite reduction in ruminal microbiota[J].Journal of Animal Science,2001,72(2):117-125.
[9]  RUSSELL J B.The importance of pH in the regulation of ruminal acetate to propionate ratio and methane production in vitro[J].Journal of Dairy Science,1998,81(12):3222-3230.
[10]  LUND P,DAHL R,YANG H J,et al.The acute effect of addition of nitrate on in vitro and in vivo methane emission in dairy cows[J].Animal Production Science,2014,54(9):1432-1435.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133