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精氨酸通过内分泌途径调控氧化应激仔猪生长相关因子基因表达

DOI: 10.3969/j.issn.1006-267x.2015.04.027

Keywords: 精氨酸,氧化应激,断奶仔猪,内分泌,生长相关因子

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Abstract:

本试验通过研究精氨酸(Arg)对氧化应激仔猪内分泌及生长相关因子基因表达的影响,探寻Arg抗氧化应激的作用机制。选择24头健康PIC断奶仔公猪,随机分为4组,每组6个重复,每个重复1头猪。各组试验猪分别饲喂基础饲粮+注射生理盐水(对照组)、基础饲粮+注射Diquat(氧化应激组)、基础饲粮+0.8%Arg+注射Diquat(0.8%Arg组)、基础饲粮+1.6%Arg+注射Diquat(1.6%Arg组)。在试验第8天,各试验组仔猪腹腔注射Diquat溶液(10mg/kgBW)诱导氧化应激,对照组仔猪注射等量的灭菌生理盐水。正式试验期共11d。结果表明:1)氧化应激显著降低了仔猪应激后24h的血浆胰岛素和胰岛素样生长因子-Ⅰ(IGF-Ⅰ)的浓度(P<0.05),补充1.6%Arg显著增加了氧化应激仔猪应激后48、96h的血浆IGF-Ⅰ浓度(P<0.05)。2)氧化应激对仔猪血浆一氧化氮合成酶的活性无显著影响(P>0.05),显著降低了仔猪肝脏一氧化氮合成酶的活性(P<0.05);补充1.6%Arg可显著抑制氧化应激导致的肝脏一氧化氮合成酶活性的降低(P<0.05)。3)氧化应激可显著降低肝脏IGF-Ⅰ、胰岛素样生长因子-Ⅰ受体(IGF-ⅠR)、胰岛素样生长因子结合蛋白3(IGFBP3)mRNA的相对表达量(P<0.05),而对肌肉中这3个基因mRNA的相对表达量无显著影响(P>0.05);补充1.6%Arg可显著增加肝脏IGF-Ⅰ、IGFBP3及肌肉IGF-Ⅰ、IGF-ⅠR和IGFBP3mRNA的相对表达量(P<0.05)。综上所述,Arg通过促进胰岛素、IGF-Ⅰ的分泌进而上调生长相关因子基因表达是其缓解仔猪氧化应激的途径之一。

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