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壳聚糖对大肠杆菌攻毒仔猪生长性能和免疫力的影响

DOI: 10.3969/j.issn.1006-267x.2011.10.019

Keywords: 壳聚糖,仔猪,生长性能,免疫力,细胞因子

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Abstract:

本试验旨在探讨饲粮中添加壳聚糖对早期断奶仔猪生长、腹泻和免疫力的影响。试验选择50头21日龄、平均体重(6.62±0.59)kg的断奶仔猪,随机分为5组,每组10个重复,每个重复1头猪。预试期采用大肠杆菌攻毒(1×109个细胞/头),对照组饲喂基础饲粮,试验组分别在基础饲粮中添加50mg/kg金霉素和200、300、400mg/kg壳聚糖。试验期21d。结果表明:1)与对照组相比,各试验组平均日采食量提高(P>0.05),料重比和腹泻率降低,平均日增重分别提高了15.8%、9.2%、16.8%和21.1%(P>0.05)。2)与对照组相比,各壳聚糖组血清免疫球蛋白G(IgG)和免疫球蛋白M(IgM)水平以及回肠黏膜白介素1β(IL-1β)、白介素6(IL-6)和肿瘤坏死因子α(TNF-α)mRNA相对表达量均升高,金霉素组均下降,其中300和400mg/kg壳聚糖组的血清IgG和IgM水平与金霉素组差异显著(P<0.05),400mg/kg壳聚糖组血清IgG水平还与对照组差异显著(P<0.05),壳聚糖组IL-1β和TNF-αmRNA相对表达量与金霉素组差异显著(P<0.05),300和400mg/kg壳聚糖组IL-6mRNA表达量与金霉素组差异显著(P<0.05)。结果提示,饲粮中添加壳聚糖能促进仔猪生长,降低腹泻率,提高非特异性免疫力,且作用效果随饲粮中壳聚糖浓度的增加有增强的趋势。

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