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锌和降钙素基因相关肽对仔猪摄食的影响及其机制研究

DOI: 10.3969/j.issn.1006-267x.2011.09.013

Keywords: ,CGRP,仔猪,采食量

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Abstract:

本试验旨在研究锌和降钙素基因相关肽(CGRP)对仔猪摄食的影响及其机制。试验选用30头9.57kg左右的“杜×长×大”三元杂交仔猪,随机分为5组(对照Ⅰ组、试验Ⅰ组、对照Ⅱ组、试验Ⅱ组和试验Ⅲ组),每组6头猪。5组均饲喂相同的基础饲粮,饲养试验期14d。禁食24h后,对照Ⅰ组、试验Ⅰ组和试验Ⅱ组分别注射0、2和4mg/kgBW的锌,注射24h后屠宰;对照Ⅱ组和试验Ⅲ组分别注射0和0.05mg/kgBW的CGRP,注射2h后屠宰。结果表明:1)与对照Ⅰ组相比,试验Ⅰ组采食量显著提高(P<0.05),试验Ⅱ组采食量却显著降低(P<0.05);与对照Ⅱ组相比,试验Ⅲ组采食量显著下降(P<0.05)。2)注射锌对仔猪血糖和瘦素水平无显著性影响(P>0.05),而使甘油三酯(TG)、胰岛素和胰高血糖素水平显著降低(P<0.05);注射CGRP显著降低血糖、TG、胰岛素水平(P<0.05),显著提高胰高血糖素水平(P<0.05),而对瘦素水平无显著性影响(P>0.05)。3)与对照Ⅰ组相比,试验Ⅰ组和试验Ⅱ组神经肽Y(NPY)mRNA表达量显著提高(P<0.05),CGRPmRNA表达量和胆囊收缩素(CCK)mRNA表达量显著降低(P<0.05);与对照Ⅱ组相比,试验Ⅲ组NPYmRNA表达量显著降低(P<0.05),CGRPmRNA表达量和CCKmRNA表达量显著提高(P<0.05)。由此可知,一定剂量的锌可通过调节胰岛素和胰高血糖素分泌,诱导食欲神经肽NPYmRNA表达,抑制饱腹神经肽CGRP和CCKmRNA表达,促进仔猪采食。CGRP可通过促进胰高血糖素分泌,抑制食欲神经肽NPYmRNA表达,诱导饱腹神经肽CGRP和CCKmRNA表达,抑制仔猪采食。

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