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十二指肠灌注亮氨酸对山羊胰腺外分泌功能的影响

DOI: 10.3969/j.issn.1006-267x.2011.09.009

Keywords: 亮氨酸,奶山羊,十二指肠灌注,胰腺外分泌,胰岛素

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Abstract:

本试验旨在研究十二指肠灌注亮氨酸(leucine,Leu)对山羊胰腺外分泌及血浆指标的影响,进而为提高反刍动物小肠淀粉利用率提供理论依据。本研究采用4×4拉丁方试验设计,选用4只周岁龄关中奶山羊,手术安装十二指肠瘘管、总胆管插管,进行十二指肠Leu灌注试验(灌注水平分别为0、3、6、9g/d),研究Leu不同灌注水平对胰腺外分泌功能、血浆葡萄糖及胰岛素浓度的影响。结果表明:十二指肠灌注Leu,血浆中的葡萄糖和胰岛素含量、胰液分泌量、胰液总蛋白质浓度及合成速率、胰蛋白酶、糜蛋白酶和脂肪酶的分泌均不受影响(P>0.05)。随Leu灌注水平的提高,胰腺α-淀粉酶的分泌浓度差异不显著(P>0.05),而分泌速率呈先升高后降低再回升的变化规律(P<0.05)。结果提示,Leu可不依赖于胰岛素直接长期调控山羊胰腺α-淀粉酶的分泌。

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