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细菌素Subticin112对小鼠的急性毒性作用研究

DOI: 10.3969/j.issn.1006-267x.2011.08.026

Keywords: 枯草芽孢杆菌素,急性毒性作用,最大耐受剂量,半数致死剂量

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Abstract:

本文旨在研究枯草芽孢杆菌素Subticin112对小鼠的急性毒性作用,并测定该细菌素单次给药的半数致死剂量(medianlethaldose,LD50)或最大耐受剂量(maximumtolerateddose,MTD)。将80只体重为(20±2)g的SPF级KM小鼠随机分成4组,雌雄各占1/2,每组每只小鼠分别灌胃0.4mL的无菌生理盐水(对照组)及相同体积、浓度分别为0.25(低浓度组)、0.50(中浓度组)和1.00g/mL(高浓度组,且为最大给药浓度)的Subticin112溶液。灌胃后14d内密切观察小鼠的反应。试验结束后记录小鼠的体重变化,眼球采血法收集小鼠血液,进行血清生化指标的测定,解剖后分别取心脏、肝脏、脾脏和肾脏等器官称重以测定其脏器系数,每组随机取2只小鼠(雌雄各1只)的心脏、肝脏、脾脏和肾脏等器官用于制作病理组织切片。给药14d后,各组小鼠均正常生长且未表现出任何中毒症状。试验组小鼠增重与对照组相比,虽然有下降的趋势,但差异不显著(P>0.05);试验组小鼠的脏器系数、血清生化指标等与对照组相比差异均不显著(P>0.05);观察试验组小鼠的心脏、肝脏、脾脏和肾脏等器官的病理组织切片也均未出现任何病变或坏死细胞。由此可见,Subticin112对小鼠的MTD大于20g/kgBW,根据急性毒性试验国家标准(GB15193.3—2003)中的急性毒性剂量分级表,Subticin112属于无毒药物。

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